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; Crooks, Antisense drug Technology: Principles, strategies and applications, 2.sup.nd Ed.
1987) Gene Transfer Vectors For Mammalian Cells, (Cold Spring Harbor Laboratory); Wu et al., eds., Methods In Enzymology, Vols. 1984) Transcription And Translation; Freshney (1987) Culture Of Animal Cells (Alan R. Liss, Inc.); Immobilized Cells And Enzymes (IRL Press) (1986); Perbal (1984) A Practical Guide To Molecular Cloning; the treatise, Methods In Enzymology (Academic Press, Inc., N.Y.); Miller and Calos eds.
1984) Oligonucleotide Synthesis; Mullis et al. Tau antisense oligonucleotide impact on spontaneous calcium oscillations in primary neurons is summarized in FIG. 4. See Murphy et al., J. Neurosci. Tau antisense oligonucleotide impact on spontaneous calcium oscillations in primary neurons is shown in FIG.
4. The oligomers in neuronal cells showing AMPA-mediated oscillations that are equal to or greater than 75% of the calcium oscillations in the untreated control cells were selected for further ***ysis. Examples of topical formulations include those in which the oligomer of the invention are in admixture with a topical delivery agent such as lipids, liposomes, fatty acids, fatty acid esters, steroids, chelating agents and surfactants.
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