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In the current examine, immunohistological and immunoblot ***yses had been used to look at the expression of the neural cell adhesion molecule (N-CAM) and the neuron-glia cell adhesion molecule (Ng-CAM) inside completely different components of a functionally linked neuromuscular system extending from skeletal muscle to the spinal cord after peripheral nerve injury.
Based on the rationale that such molecules include a sign sequence as a way to be introduced on the cell floor, we've employed a eukaryotic-based mostly, sign-sequence-lure strategy to identify novel secreted and membrane-bound molecules which are expressed in myelinating and non-myelinating Schwann cells.
Our outcomes point out that myelinating Schwann cells express a battery of novel CAMs that may mediate their interactions with the underlying axons. Using cDNA libraries derived from dbcAMP-stimulated major Schwann cells and 3-day-outdated rat sciatic nerve mRNAs, we generated an in depth record of novel molecules expressed in myelinating nerves within the PNS.
Anti-N-CAM antibodies stained neurites, Schwann cells, and the perineurium of the regenerating sciatic nerve.